Ghana received an invitation from Cricket South Africa to take part for the first time in the expanded tournament.
However, the West Africa nation cited logistical constraints as the reason to pull out.
“It is disappointing that Ghana had to pull out of the competition,” said International Cricket Council Africa development officer Justine Ligyalingi.
“As it was going to be their debut at this tournament, it would have been a great experience for their national team, but we are glad to confirm the involvement of another debutant in Uganda.
The preliminary round of the tourney is scheduled to come off between September 14 and September 16.
The teams to take part are Kenya, Namibia, Nigeria, Zimbabwe, and now Uganda.
Hosts South Africa will be represented by 13 provincial teams.